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Liquid Media in 2ml Ampoules

Items listed must be REFRIGERATED and SHIP VIA OVERNIGHT directly from the manufacturer. Manufacturer's minimum order quantity applies. All items in the section that are added to an on-line order, will be placed on hold to verify manufacturers minimums and correct shipping method.

Cetrimide Broth
Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water soluble, non-fluorescent, phenazine pigment) which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth.

Cetrimide (N-cetyl-NNN-trimethylammonium bromide) is added to inhibit bacteria other than Pseudomonas aeruginosa. Its action as a quaternary ammonium cationic detergent causes nitrogen and phosphorous to be released from bacterial cells other than Pseudomonas aeruginosa.

Enterococcus Broth
Enterococcus broth is a modified version of the improved media described by Slanetz and Bartley with TTC. The membrane filtration method is simple to perform, does not require confirmation and permits a direct count of enterococci in 48 hours.

HPC Broth with TTC
HPC is used to determine total count at incubation temperatures of 35º C. All bacteria develop on HPC with indicator media and produce a red color as a result of the precipitation of formazan following the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by bacteria.

KF-Streptococcus Broth
KF-Streptococcus Broth is selective for the determination of fecal streptococci in polluted surface waters. Maltose and lactose are fermentable carbohydrates, sodium azide is the selective agent and brom cresol purple is the indicator dye.

Mannitol Salt Broth
Because of the amount of peptones and beef extract, Mannitol Salt is a nutrient rich medium. Most bacteria (other than staphylococci) are inhibited by the high concentration of sodium chloride. Organisms capable of fermenting mannitol, e.g. Staphylococcus aureus, cause a pH change in the media. With phenol red as the pH indicator the colonies appear with a yellow coloration.

Membrane Lauryl Sulfate Broth
This media was developed for the detection of coliform organisms and is now the media of choice for the enumeration of total coliforms and Escherichia coli in the United Kingdom. This media replaced membrane enriched broth containing 0.4% Teepol 610.

M-Endo Coliform Broth
M-Endo is a red colored media, which needs to be stored in the dark to prevent discoloration. Gram-positive bacteria are inhibited on this media by the desoxycholate and lauryl sulfate. The addition of ethanol increases the antibacterial nature of the formulation. Lactose fermenting organisms form aldehydes, which react with Schiff’s reagent (basic fuchsin and sodium sulfite) to give red colored zones around the colonies. Coliform colonies are therefore red with a characteristic metallic sheen.

M-FC Broth
Allows the development of fecal coliforms at elevated temperatures (44.5° C).

M-FC with Rosolic Acid
M-FC with Rosolic Acid acts and functions in the same way as m-FC Broth. Rosolic acid inhibits bacterial growth in general, except for fecal coliforms.

M-Green Select Broth
M-Green Select Broth is an improved modification of the liquid media, m-Green Yeast and Mold Broth and was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low pH, which inhibits bacterial growth. The addition of Chloramphenicol further inhibits the growth of bacteria to allow for the development and enumeration of yeast and mold. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the pH which aids in the inhibition of bacterial growth, but also produces an acid reaction that causes residual bromocresol green to change to yellow.

M-Green Yeast and Mold
M-Green is an improved modification of the liquid media, m-Yeast and Mold Broth and was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low pH, which inhibits bacterial growth. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the pH which aids in the inhibition of bacterial growth, but also produces an acid reaction that causes residual bromocresol green to change to yellow.

M-TGE Total Count Media
All bacteria develop on TGE media and produce a range of different colored and sized colonies.


Orange Serum Media
Organisms known to grow in single strength and concentrated juices are lactic acid and acetic acid bacteria and yeast. Lactobacilli, Leuconostoc and yeast have all been identified as spoilage organisms by numerous authors. Orange serum at pH 5.4 to 5.6 has been reported to yield maximum counts of all types of spoilage organisms in mixed cultures and in single culture comparison tests.

Pseudomonas Broth
Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water soluble, non-fluorescent, phenazine pigment) which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than pseudomonads. Glycerol serves as both an energy source and helps in the promotion of pyocyanin.

R2 Broth
R2 broth can be used to determine heterotrophic plate count at 35º C. When incubated at lower temperatures (25–30º C) for longer periods of 72–96 hours, it can also be used to recover environmentally stressed organisms, or those that are chlorine tolerant.

Total Count Media with TTC
All bacteria develop on Total Count Media with indicator and produce a red color as a result of the precipitation of formazan following the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by bacteria.

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD)
Use of the medium at pH 5.5 and incubation at 25° C will give reliable counts for brewer’s yeast. Adjustment of the pH to 6.5 and incubation at 30° C allows for the selective growth of baker’s and distiller’s yeast.

Click on Item# to view possible quantity discounts that may be available.
    • Item#
    • Description
    • Price
    • Quantity
    • AKM-9009-0005
    • 2ml ampoule Cetrimide Broth, Case of 50 - 5 Case Manufacturer's Minimum Order
    • $110.92
    • AKM-9009-0011
    • 2ml ampoule Enterococcus Broth, Case of 50 - 5 Case Manufacturer's Minimum Order
    • $110.77
    • AKM-9009-0009
    • 2ml ampoule HPC Broth with TTC, Case of 50 - 5 Case Manufacturer's Minimum Order
    • $97.79
    • AKM-9009-0012
    • 2ml ampoule KF-Streptococcus Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $73.85
    • AKM-9009-0013
    • 2ml ampoule Mannitol Salt Broth, Case of 50 - 5 Case Manufacturer's Minimum Order
    • $102.64
    • AKM-9009-0003
    • 2ml ampoule M-Endo Coliform Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0008
    • 2ml ampoule M-FC Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $82.47
    • AKM-9009-0015
    • 2ml ampoule M-FC Broth with Rosolic Acid, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $73.85
    • AKM-9009-0016
    • 2ml ampoule M-Green Select Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $90.49
    • AKM-9009-0001
    • 2ml ampoule M-Green Yeast & Mold Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0002
    • 2ml ampoule M-TGE Total Count Media, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0017
    • 2ml ampoule Orange Serum Media, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0018
    • 2ml ampoule Pseudomonas Broth, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0020
    • 2ml ampoule Total Count TTC Indicator, Case of 50 - 6 Case Manufacturer's Minimum Order
    • $79.81
    • AKM-9009-0022
    • 2ml ampoule Wallerstein Nutrient Broth (WL), Case of 50 - 5 Case Manufacturer's Minimum Order
    • $228.82
    • AKM-9009-0021
    • 2ml ampoule Wallerstein Differential Broth (WLD), Case of 50 - 5 Case Manufacturer's Minimum Order
    • $95.51
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