Items listed must be REFRIGERATED and SHIP VIA OVERNIGHT directly from the manufacturer. Manufacturer's minimum order quantity applies. All items in the section that are added to an on-line order, will be placed on hold to verify manufacturers minimums and correct shipping method.
MI Broth and MI Agar
MI Broth detects the presence of coliform bacteria by the production of b-galactosidase, which cleaves the substrate MUGal to produce 4-Methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The b-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces b-galactosidase, it will also fluoresce. The antibiotic cefsulodin is added to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.
Trypticase Soy Broth – Single Strength
General-purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms. Trypticase Soy Broth – Single Strength complies with the demands of the DIN Norm 10167 for the detection of Escherichia coli serotype 0157:H7 in foods and FDA-BAM for the isolation of enterohemorrhagic Escherichia coli (EHEC). In addition the media conforms to the formula of the U.S. Pharmacopoeia.
Trypticase Soy Broth – Double Strength
TSB broth is a medium that will support the growth of a wide variety of microorganisms including aerobic, facultative and anaerobic bacteria and fungi.